Category:Protocols: Difference between revisions

General Maintenance

• Changing the high pressure dewar
• Filling a Magnet with Nitrogen
• Autoclaving Laboratory Glassware and Media
• Chemical Disinfection of Glassware
• Requesting Balance Calibration
• Requesting Pipette Calibration
• Using the UV-Vis
• Using and Maintaining pH Meter
• -80 Freezer Storage and Maintenance
• Freeze Dryer Maintenance
• Lab Notebook Guidelines
• Sample Barcoding

Protein Preparation

• Buffer Exchange and Solution Concentration
• Finding a Protein Target on the NESG website
• Choosing a Plasmid
• Plasmid Purification and Transformation Protocol
• Creating Stock Cultures of Bacteria
• Luria-Bertani Media
• M9 Minimal Media
• Protein Overexpression and Extraction
• SDS-PAGE Protocol
• Running a Cobalt Affinity Column
• Dialysis
• Centrifugal Protein Concentration and Buffer Exchange
• Using the Stirred Cell Concentrator

Data Collection

• Gap Sampling
• Water Suppression with presaturation pulses (zgpr/zgcppr
• Non-uniform Sampling
• Collecting a 15N Edited HSQC
• Collecting CEST Data

Data Processing and Analysis

• Analysis of 1D Line-Broadening Screen
• FastModelFree
• 2D NMR Analysis (CCPNMR)
• 1H NMR Analysis (SIMCA)
• 1H NMR Analysis (ACDLab)
• Processing CEST Data
• Titration Data Analysis in nmrPipe
• Non-Uniform Sampling
• NMRFAM-SPARKY Guide
• NMRFAM-SPARKY: Automated Peak Assignment
• 2D NMR Processing in Linux and Windows
• 1D NMR Processing in Linux and Windows Example Script
• Sample Collection and Processing for Protein Backbone Assignments
• Example Scripts for NMRPipe Processing

Miscellaneous

• Agarose Gel
• 700 MHz NMR checklist
• 500 MHz NMR checklist
• 1D Macro
• Setting Up a Virtual Screen with AutoDock
• Simple Protein Crosslinking
• 1D NMR Titrations

Cell Culturing

• Cell Culturing Dr. Franco Lab

Metabolomics

• Metabolite Extraction
• MetPa for metabolomics
• Making Heatmaps
• One way ANOVA in R
• P-Value adjustment for multiple comparisons
• PCA-Utils
• NMR Tube Deep Cleaning
• Noise Removal for PCA
• Weighted Linear Least Squares
• Serum Preparation for 1D NMR
• Whole Blood Preparation for 1D NMR
• Urine Preparation for 1D NMR
• Cell Culture Preparation for 1D NMR
• LC-MS metabolomics guide-CIBC collaborations

Safety and Inspections

• Lab Responsibilities
• Inspection Checklist
• Safety Contacts, Resources

Demos

• Peroxide Clock
• Traffic Light Reaction
• Orange Juice Clock
• Gummy Bear Freeze
• Lava Lamps
• Batteries
• Rocket Launcher
• Women in Science: Checklist
• Maxey Day: Checklist