Category:Protocols: Difference between revisions
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*[[-80 Freezer Storage and Maintenance]] | *[[-80 Freezer Storage and Maintenance]] | ||
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*[[Lab Notebook Guidelines]] | |||
''Protein Preparation'' | ''Protein Preparation'' | ||
Revision as of 22:17, 30 September 2019
General Maintenance
- Changing the high pressure dewar
- Filling a Magnet with Nitrogen
- Autoclaving Laboratory Glassware and Media
- Chemical Disinfection of Glassware
- Requesting Balance Calibration
- Requesting Pipette Calibration
- Using the UV-Vis
- Using and Maintaining pH Meter
- -80 Freezer Storage and Maintenance
- Freeze Dryer Maintenance
- Lab Notebook Guidelines
Protein Preparation
- Buffer Exchange and Solution Concentration
- Finding a Protein Target on the NESG website
- Choosing a Plasmid
- Plasmid Purification and Transformation Protocol
- Creating Stock Cultures of Bacteria
- Luria-Bertani Media
- M9 Minimal Media
- Protein Overexpression and Extraction
- SDS-PAGE Protocol
- Running a Cobalt Affinity Column
- Dialysis
- Centrifugal Protein Concentration and Buffer Exchange
- Using the Stirred Cell Concentrator
Data Collection
- Gap Sampling
- Water Suppression with presaturation pulses (zgpr/zgcppr
- Non-uniform Sampling
- Collecting a 15N Edited HSQC
- Collecting CEST Data
Data Processing and Analysis
- Analysis of 1D Line-Broadening Screen
- FastModelFree
- 2D NMR Analysis (CCPNMR)
- 1H NMR Analysis (SIMCA)
- 1H NMR Analysis (ACDLab)
- Processing CEST Data
- Titration Data Analysis in nmrPipe
- Non-Uniform Sampling
- NMRFAM-SPARKY Guide
- NMRFAM-SPARKY: Automated Peak Assignment
- NMR Processing in Linux and Windows
- Sample Collection and Processing for Protein Backbone Assignments
- Example Scripts for NMRPipe Processing
Miscellaneous
- Agarose Gel
- 700 MHz NMR checklist
- 500 MHz NMR checklist
- 1D Macro
- Setting Up a Virtual Screen with AutoDock
- Simple Protein Crosslinking
- 1D NMR Titrations
Cell Culturing
Metabolomics
- MetPa for metabolomics
- Making Heatmaps
- Metabolite Extraction
- One way ANOVA in R
- P-Value adjustment for multiple comparisons
- PCA-Utils
- NMR Tube Deep Cleaning
- Noise Removal for PCA
- Weighted Linear Least Squares
- Serum Preparation for 1D NMR
- Urine Preparation for 1D NMR
Safety and Inspections
Demos
Subcategories
This category has the following 22 subcategories, out of 22 total.
A
- AutoDock (2 P)
B
- Bioscreen (4 P)
C
- Cell Culturing (16 P)
- Computer Simulation (4 P)
D
- Demos (12 P)
E
F
- FAST-NMR (6 P)
G
- Gel (2 P)
- General Maintenance (14 P)
L
- Lab Safety (14 P)
M
- Molecular Docking (1 P)
- MVAPACK (3 P)
N
- Needs Updating (25 P)
P
- Proteomics (empty)
S
- Sample Preparation (9 P)
T
- Troubleshooting (2 P)
U
- Update Wiki (3 P)