User contributions for Jcatazaro

14 March 2017

• 21:2821:28, 14 March 2017 diff hist +1‎ Collecting CEST Data ‎No edit summary
• 21:2821:28, 14 March 2017 diff hist +1,572‎ N Collecting CEST Data ‎ Created page with "Collecting CEST Data # Open TopSpin >= 3.2.X # Edit temperature ##“edte” # Create a dataset and collect a NHSQC to check resolution and NS # Type “edpy”, select n15c..."
• 21:2521:25, 14 March 2017 diff hist +26‎ Category:Protocols ‎No edit summary
• 21:2421:24, 14 March 2017 diff hist +778‎ N Simple Protein Crosslinking ‎ Created page with "Simple Protein Crosslinking # Have tagged, purified protein ready before starting the rest of this procedure. # Grow up a culture of target cells (500mL is fine). # Spin dow..."
• 21:2321:23, 14 March 2017 diff hist +33‎ Category:Protocols ‎No edit summary
• 21:2321:23, 14 March 2017 diff hist +1,295‎ N Setting Up a Virtual Screen with AutoDock ‎ Created page with "Setting Up a Virtual Screen with AutoDock # Install MGLTools and AutoDock, download raccoon.py # Navigate to the MGLTools directory ## "cd /Library/MGLTools/1.5.6/bin" # Ope..." current
• 21:2121:21, 14 March 2017 diff hist +47‎ Category:Protocols ‎No edit summary
• 21:1921:19, 14 March 2017 diff hist +76‎ Titration Data Analysis in nmrPipe ‎No edit summary
• 21:1821:18, 14 March 2017 diff hist +1,083‎ N Titration Data Analysis in nmrPipe ‎ Created page with "Titration Data Analysis in nmrPipe 1. Type “csh” in your terminal to get a c-shell 2. In a directory that contains the data folders create an “in.list” file which ..."
• 21:1721:17, 14 March 2017 diff hist +40‎ Category:Protocols ‎No edit summary
• 21:1621:16, 14 March 2017 diff hist +1,476‎ N Collecting a 15N Edited HSQC ‎ Created page with "Collecting a Superb 15N edited HSQC (NHSQC) # Concentrate your protein as much as possible. It should be at least 50uM for 2D work and around 1mM for 3D work. # Spike in 10% ..."
• 21:1121:11, 14 March 2017 diff hist +34‎ Category:Protocols ‎No edit summary
• 21:0321:03, 14 March 2017 diff hist +1,945‎ N Using the Stirred Cell Concentrator ‎ Created page with "Using the Stirred Cell Concentrator # Obtain a 44 mm membrane for the concentrator. Handle the membrane by touching only its edges. # Place the membrane, shiny side down, in..."
• 21:0121:01, 14 March 2017 diff hist +41‎ Category:Protocols ‎No edit summary
• 21:0021:00, 14 March 2017 diff hist +940‎ N Centrifugal Protein Concentration and Buffer Exchange ‎ Created page with "Centrifugal Protein Concentration/Buffer Exchange # Obtain a centrifugal filter (Amicon or Centricon) with a molecular weight cutoff at least half of your protein’s weight...."
• 20:5920:59, 14 March 2017 diff hist +59‎ Category:Protocols ‎No edit summary
• 20:5820:58, 14 March 2017 diff hist +1‎ Dialysis ‎No edit summary
• 20:5820:58, 14 March 2017 diff hist +895‎ N Dialysis ‎ Created page with "Dialysis # Grab enough dialysis tubing (or a Float-a-lyzer) to hold your sample. If using tubing, make sure there is extra tubing to fold over and clip. # Fold one end of the..."
• 20:5720:57, 14 March 2017 diff hist +14‎ Category:Protocols ‎No edit summary
• 20:5620:56, 14 March 2017 diff hist +1,301‎ N Running a Cobalt Affinity Column ‎ Created page with "Running a Cobalt Affinity Column (Purifying a His-tagged Protein) # Obtain the cobalt resin from the refrigerator and a clean column with a valve and top. # Swirl the cobalt..."
• 20:5520:55, 14 March 2017 diff hist +38‎ Category:Protocols ‎No edit summary
• 20:5320:53, 14 March 2017 diff hist +23‎ Category:Protocols ‎No edit summary
• 20:2320:23, 14 March 2017 diff hist −3‎ Protein Overexpression and Extraction ‎No edit summary
• 20:2220:22, 14 March 2017 diff hist 0‎ Protein Overexpression and Extraction ‎No edit summary
• 20:2120:21, 14 March 2017 diff hist +6‎ Protein Overexpression and Extraction ‎No edit summary
• 20:1920:19, 14 March 2017 diff hist +1‎ Protein Overexpression and Extraction ‎No edit summary
• 20:1820:18, 14 March 2017 diff hist +1,796‎ N Protein Overexpression and Extraction ‎ Created page with "Protein Overexpression and Extraction # Prepare and sterilize a minimal media where the carbon and nitrogen source can be controlled (M9 media). Make sure the volume of the m..."
• 20:1320:13, 14 March 2017 diff hist +43‎ Category:Protocols ‎No edit summary
• 20:1020:10, 14 March 2017 diff hist +423‎ N M9 Minimal Media ‎ Created page with "M9 Minimal Media Per liter of sterile water: *5X M9 salts* 200 mL *1M MgSO4 2 mL *20% glucose** 20 mL *1M CaCl2 0.1 mL 5X M9 salts Per liter: *Na2HPO4 64 grams *KH..."
• 20:0920:09, 14 March 2017 diff hist +22‎ Category:Protocols ‎No edit summary
• 20:0820:08, 14 March 2017 diff hist +3‎ Luria-Bertani Media ‎No edit summary
• 20:0820:08, 14 March 2017 diff hist +142‎ N Luria-Bertani Media ‎ Created page with "Luria-Bertani Media Per liter: Tryptone (Shelf A) 10 grams Yeast Extract (Shelf A) 5 grams NaCl (Shelf A) 10 grams Adjust to desired pH."
• 20:0720:07, 14 March 2017 diff hist +25‎ Category:Protocols ‎No edit summary
• 20:0520:05, 14 March 2017 diff hist +630‎ N Creating Stock Cultures of Bacteria ‎ Created page with "Creating Stock Cultures of Bacteria # Heat an appropriate media (usually complex like LB, 100 mL) up to 37°C in an incubator. # Inoculate the media with bacteria. This is us..."
• 20:0420:04, 14 March 2017 diff hist +41‎ Category:Protocols ‎No edit summary
• 20:0220:02, 14 March 2017 diff hist −44‎ Plasmid Purification and Transformation Protocol ‎No edit summary
• 20:0120:01, 14 March 2017 diff hist −2‎ Plasmid Purification and Transformation Protocol ‎No edit summary
• 19:5919:59, 14 March 2017 diff hist +1,849‎ N Plasmid Purification and Transformation Protocol ‎ Created page with "Plasmid Purification/Transformation Protocol – JC 02/18/2016 Adapted from protocols found at “http://web.mnstate.edu/provost/” # Expand culture of bacteria overnight in..."
• 19:5719:57, 14 March 2017 diff hist +54‎ Category:Protocols ‎No edit summary
• 19:5619:56, 14 March 2017 diff hist +453‎ N Choosing a Plasmid ‎ Created page with "Choosing a Plasmid This step is necessary if your protein target does not come with a designated plasmid. Instead of steps, these are requirements for what should be located ..."
• 19:5519:55, 14 March 2017 diff hist +24‎ Category:Protocols ‎No edit summary
• 19:5419:54, 14 March 2017 diff hist +921‎ N Finding a Protein Target on the NESG website ‎ Created page with "Finding a Protein Target (on the NESG website) # Login: proteins Password: 2study # Start by focusing your search on a particular organism: human, myco, staph… # Begin by cl..."
• 19:5219:52, 14 March 2017 diff hist +50‎ Category:Protocols ‎No edit summary
• 19:5119:51, 14 March 2017 diff hist +478‎ N Chemical Disinfection of Glassware ‎ Created page with "Chemical Disinfection of Glassware Glassware and any other non-disposable item that comes into contact with biohazardous material (anything living) must be chemically disinfe..."
• 19:5019:50, 14 March 2017 diff hist +40‎ Category:Protocols ‎No edit summary
• 19:4919:49, 14 March 2017 diff hist −9‎ Autoclaving Laboratory Glassware and Media ‎No edit summary
• 19:4719:47, 14 March 2017 diff hist +1,788‎ N Autoclaving Laboratory Glassware and Media ‎ Created page with "Autoclaving Laboratory Glassware (and Media) Glassware that is to be used for cell growth must be sterilized beforehand. This is accomplished by first chemically disinfecting..."
• 19:4619:46, 14 March 2017 diff hist +1,239‎ N Filling a Magnet with Nitrogen ‎ Created page with "Filling a Magnet with Nitrogen # Find a rubber transfer line, two additional short pieces of rubber, and a glove. # Wheel the low pressure dewar up to the magnet to be filled..."
• 19:4419:44, 14 March 2017 diff hist +48‎ Category:Protocols ‎No edit summary
• 19:4419:44, 14 March 2017 diff hist +2‎ Category:Protocols ‎No edit summary