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• 19:52, 21 July 2022 Wiki Administrator talk contribs created page Culture Passage Cells (Created page with "==Culture and passage cells== * ready to passage when cell confluence to 80% or 90% or when the cell density reaches an average of 2 X 105 cells/cm2 * remove medium by aspiration * wash cells with PBS to remove the residual medium * add Trypsin-EDTA (enough to cover the flask surface) * incubate at 37oC for 5 minutes * observe by microscope to see cell death * add 2ml of medium trypsinization * pipet to detach all cells * transfer cell suspension to 15 ml tube...")