User contributions for Jcatazaro

14 March 2017

• 21:0121:01, 14 March 2017 diff hist +41‎ Category:Protocols ‎No edit summary
• 21:0021:00, 14 March 2017 diff hist +940‎ N Centrifugal Protein Concentration and Buffer Exchange ‎ Created page with "Centrifugal Protein Concentration/Buffer Exchange # Obtain a centrifugal filter (Amicon or Centricon) with a molecular weight cutoff at least half of your protein’s weight...."
• 20:5920:59, 14 March 2017 diff hist +59‎ Category:Protocols ‎No edit summary
• 20:5820:58, 14 March 2017 diff hist +1‎ Dialysis ‎No edit summary
• 20:5820:58, 14 March 2017 diff hist +895‎ N Dialysis ‎ Created page with "Dialysis # Grab enough dialysis tubing (or a Float-a-lyzer) to hold your sample. If using tubing, make sure there is extra tubing to fold over and clip. # Fold one end of the..."
• 20:5720:57, 14 March 2017 diff hist +14‎ Category:Protocols ‎No edit summary
• 20:5620:56, 14 March 2017 diff hist +1,301‎ N Running a Cobalt Affinity Column ‎ Created page with "Running a Cobalt Affinity Column (Purifying a His-tagged Protein) # Obtain the cobalt resin from the refrigerator and a clean column with a valve and top. # Swirl the cobalt..."
• 20:5520:55, 14 March 2017 diff hist +38‎ Category:Protocols ‎No edit summary
• 20:5320:53, 14 March 2017 diff hist +23‎ Category:Protocols ‎No edit summary
• 20:2320:23, 14 March 2017 diff hist −3‎ Protein Overexpression and Extraction ‎No edit summary
• 20:2220:22, 14 March 2017 diff hist 0‎ Protein Overexpression and Extraction ‎No edit summary
• 20:2120:21, 14 March 2017 diff hist +6‎ Protein Overexpression and Extraction ‎No edit summary
• 20:1920:19, 14 March 2017 diff hist +1‎ Protein Overexpression and Extraction ‎No edit summary
• 20:1820:18, 14 March 2017 diff hist +1,796‎ N Protein Overexpression and Extraction ‎ Created page with "Protein Overexpression and Extraction # Prepare and sterilize a minimal media where the carbon and nitrogen source can be controlled (M9 media). Make sure the volume of the m..."
• 20:1320:13, 14 March 2017 diff hist +43‎ Category:Protocols ‎No edit summary
• 20:1020:10, 14 March 2017 diff hist +423‎ N M9 Minimal Media ‎ Created page with "M9 Minimal Media Per liter of sterile water: *5X M9 salts* 200 mL *1M MgSO4 2 mL *20% glucose** 20 mL *1M CaCl2 0.1 mL 5X M9 salts Per liter: *Na2HPO4 64 grams *KH..."
• 20:0920:09, 14 March 2017 diff hist +22‎ Category:Protocols ‎No edit summary
• 20:0820:08, 14 March 2017 diff hist +3‎ Luria-Bertani Media ‎No edit summary
• 20:0820:08, 14 March 2017 diff hist +142‎ N Luria-Bertani Media ‎ Created page with "Luria-Bertani Media Per liter: Tryptone (Shelf A) 10 grams Yeast Extract (Shelf A) 5 grams NaCl (Shelf A) 10 grams Adjust to desired pH."
• 20:0720:07, 14 March 2017 diff hist +25‎ Category:Protocols ‎No edit summary
• 20:0520:05, 14 March 2017 diff hist +630‎ N Creating Stock Cultures of Bacteria ‎ Created page with "Creating Stock Cultures of Bacteria # Heat an appropriate media (usually complex like LB, 100 mL) up to 37°C in an incubator. # Inoculate the media with bacteria. This is us..."
• 20:0420:04, 14 March 2017 diff hist +41‎ Category:Protocols ‎No edit summary
• 20:0220:02, 14 March 2017 diff hist −44‎ Plasmid Purification and Transformation Protocol ‎No edit summary
• 20:0120:01, 14 March 2017 diff hist −2‎ Plasmid Purification and Transformation Protocol ‎No edit summary
• 19:5919:59, 14 March 2017 diff hist +1,849‎ N Plasmid Purification and Transformation Protocol ‎ Created page with "Plasmid Purification/Transformation Protocol – JC 02/18/2016 Adapted from protocols found at “http://web.mnstate.edu/provost/” # Expand culture of bacteria overnight in..."
• 19:5719:57, 14 March 2017 diff hist +54‎ Category:Protocols ‎No edit summary
• 19:5619:56, 14 March 2017 diff hist +453‎ N Choosing a Plasmid ‎ Created page with "Choosing a Plasmid This step is necessary if your protein target does not come with a designated plasmid. Instead of steps, these are requirements for what should be located ..."
• 19:5519:55, 14 March 2017 diff hist +24‎ Category:Protocols ‎No edit summary
• 19:5419:54, 14 March 2017 diff hist +921‎ N Finding a Protein Target on the NESG website ‎ Created page with "Finding a Protein Target (on the NESG website) # Login: proteins Password: 2study # Start by focusing your search on a particular organism: human, myco, staph… # Begin by cl..."
• 19:5219:52, 14 March 2017 diff hist +50‎ Category:Protocols ‎No edit summary
• 19:5119:51, 14 March 2017 diff hist +478‎ N Chemical Disinfection of Glassware ‎ Created page with "Chemical Disinfection of Glassware Glassware and any other non-disposable item that comes into contact with biohazardous material (anything living) must be chemically disinfe..."
• 19:5019:50, 14 March 2017 diff hist +40‎ Category:Protocols ‎No edit summary
• 19:4919:49, 14 March 2017 diff hist −9‎ Autoclaving Laboratory Glassware and Media ‎No edit summary
• 19:4719:47, 14 March 2017 diff hist +1,788‎ N Autoclaving Laboratory Glassware and Media ‎ Created page with "Autoclaving Laboratory Glassware (and Media) Glassware that is to be used for cell growth must be sterilized beforehand. This is accomplished by first chemically disinfecting..."
• 19:4619:46, 14 March 2017 diff hist +1,239‎ N Filling a Magnet with Nitrogen ‎ Created page with "Filling a Magnet with Nitrogen # Find a rubber transfer line, two additional short pieces of rubber, and a glove. # Wheel the low pressure dewar up to the magnet to be filled..."
• 19:4419:44, 14 March 2017 diff hist +48‎ Category:Protocols ‎No edit summary
• 19:4419:44, 14 March 2017 diff hist +2‎ Category:Protocols ‎No edit summary
• 19:4219:42, 14 March 2017 diff hist +35‎ Category:Protocols ‎No edit summary
• 19:4119:41, 14 March 2017 diff hist −7‎ Changing the high pressure dewar ‎No edit summary
• 19:4019:40, 14 March 2017 diff hist +483‎ N Changing the high pressure dewar ‎ Created page with "Changing the High Pressure Dewar (on the 700) 1. Make sure that an experiment is not being collected on the 700. 2. Close the gas valve on the old/empty dewar. 3. Close the t..."
• 19:3819:38, 14 March 2017 diff hist +36‎ Category:Protocols ‎No edit summary
• 19:3819:38, 14 March 2017 diff hist −9‎ Gap sampling ‎No edit summary
• 19:3719:37, 14 March 2017 diff hist +9‎ Gap sampling ‎No edit summary
• 19:3519:35, 14 March 2017 diff hist −8‎ Category:Protocols ‎ Blanked the page
• 19:3519:35, 14 March 2017 diff hist −22‎ Test ‎ Blanked the page
• 19:3419:34, 14 March 2017 diff hist +22‎ N Test ‎ Created page with "category:Protocols"
• 19:3119:31, 14 March 2017 diff hist +8‎ Category:Protocols ‎No edit summary
• 19:2819:28, 14 March 2017 diff hist −37‎ Category:Protocols ‎ Blanked the page
• 19:2819:28, 14 March 2017 diff hist +37‎ Category:Protocols ‎No edit summary
• 18:4418:44, 14 March 2017 diff hist +49‎ N File:Changing the High Pressure Dewar.docx ‎ Instructions for changing the high pressure dewar current