Urine Preparation for 1D NMR: Difference between revisions
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Note: Methanol extration used in serum preperation can be used to minimize protein content and urea peak in NMR analysis. | Note: Methanol extration used in serum preperation can be used to minimize protein content and urea peak in NMR analysis. | ||
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Revision as of 04:02, 11 September 2021
1. Urine samples are aliquoted and centrifuged at 14,000 × rpm for 10 min to pellet debris
2. Supernatant samples are collected and snap frozen in Liquid Nitrogen and freeze dried to remove methanol by speed vacuum centrifugation (SpeedVac R Plus, Savant) and water by lyophilization using FreeZone™ (Labconco, Kansas City, MO) for 24 hours and stored at -20°C until NMR and MS analysis.
Note: Methanol extration used in serum preperation can be used to minimize protein content and urea peak in NMR analysis.