Creating Stock Cultures of Bacteria: Difference between revisions
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(Created page with "Creating Stock Cultures of Bacteria # Heat an appropriate media (usually complex like LB, 100 mL) up to 37°C in an incubator. # Inoculate the media with bacteria. This is us...") |
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# Add glycerol to each tube (30-50%) and vortex until thoroughly mixed. | # Add glycerol to each tube (30-50%) and vortex until thoroughly mixed. | ||
# Place all tubes into the -80°C freezer for long term storage. | # Place all tubes into the -80°C freezer for long term storage. | ||
[[category:Protocols|Protocols]] | |||
[[category:Protein_Preparation]] | |||
[[category:Cell_Culturing]] | |||
Revision as of 03:08, 11 September 2021
Creating Stock Cultures of Bacteria
- Heat an appropriate media (usually complex like LB, 100 mL) up to 37°C in an incubator.
- Inoculate the media with bacteria. This is usually done from a colony on a plate or from a stock culture.
- Start shaking the media in the incubator after inoculation.
- Allow the culture to grow to a visibly high density (4-8 hours).
- Grab a bunch of 1-2mL microcentrifuge tubes and glycerol.
- Pipet 0.5 mL of the dense culture into each microcentrifuge tube.
- Add glycerol to each tube (30-50%) and vortex until thoroughly mixed.
- Place all tubes into the -80°C freezer for long term storage.