Category:Protocols: Difference between revisions
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*[[Centrifugal Protein Concentration and Buffer Exchange]] | *[[Centrifugal Protein Concentration and Buffer Exchange]] | ||
*[[Using the Stirred Cell Concentrator]] | *[[Using the Stirred Cell Concentrator]] | ||
''Data Collection'' | ''Data Collection'' | ||
*[[Gap Sampling]] | |||
*[[Water Suppression with presaturation pulses (zgpr/zgcppr]] | |||
*[[Non-uniform Sampling] | |||
*[[Collecting a 15N Edited HSQC]] | *[[Collecting a 15N Edited HSQC]] | ||
*[[Collecting CEST Data]] | *[[Collecting CEST Data]] | ||
''Data Processing and Analysis'' | ''Data Processing and Analysis'' | ||
*[[Analysis of 1D Line-Broadening Screen]] | *[[Analysis of 1D Line-Broadening Screen]] | ||
| Line 35: | Line 40: | ||
*[[Processing CEST Data]] | *[[Processing CEST Data]] | ||
*[[Titration Data Analysis in nmrPipe]] | *[[Titration Data Analysis in nmrPipe]] | ||
*[[Non-Uniform Sampling]] | |||
*[NMRFAM-SPARKY Guide]] | |||
*[[NMRFAM-SPARKY: Automated Peak Assignment]] | |||
*[[NMR Processing in Linux and Windows]] | |||
''Miscellaneous'' | ''Miscellaneous'' | ||
*[[Agarose Gel]] | *[[Agarose Gel]] | ||
| Line 43: | Line 53: | ||
*[[Simple Protein Crosslinking]] | *[[Simple Protein Crosslinking]] | ||
*[[1D NMR Titrations]] | *[[1D NMR Titrations]] | ||
Cell Culturing | |||
*[[Cell Culturing Dr. Franco Lab]] | |||
''Metabolomics'' | |||
*[[MetPa for metabolomics]] | |||
*[[Making Heatmaps]] | |||
*[[Metabolite Extraction]] | |||
*[[One way ANOVA in R]] | |||
*[[P-Value adjustment for multiple comparisons]] | |||
*[[PCA-Utils]] | |||
*[[NMR Tube Deep Cleaning]] | |||
*[[Noise Removal for PCA]] | |||
*[[Weighted Linear Least Squares]] | |||
*[[Serum Preparation for 1D NMR]] | |||
*[[Urine Preparation for 1D NMR]] | |||
''Safety and Inspections'' | |||
*[[Lab Responsibilities]] | |||
*[[Inspection Checklist]] | |||
*[[Safety Contacts, Resources]] | |||
''Demos'' | |||
*[[Peroxide Clock]] | |||
*[[Traffic Light Reaction]] | |||
*[[Orange Juice Clock]] | |||
*[[Gummy Bear Freeze]] | |||
*[[Batteries]] | |||
*[[Rocket Launcher]] | |||
*[[Women in Science: Checklist]] | |||
*[[Maxey Day: Checklist]] | |||
Revision as of 17:35, 23 July 2019
General Maintenance
- Changing the high pressure dewar
- Filling a Magnet with Nitrogen
- Autoclaving Laboratory Glassware and Media
- Chemical Disinfection of Glassware
- Requesting Balance Calibration
- Requesting Pipette Calibration
- Using the UV-Vis
- Using/Maintaining pH Meter
Protein Preparation
- Buffer Exchange and Solution Concentration
- Finding a Protein Target on the NESG website
- Choosing a Plasmid
- Plasmid Purification and Transformation Protocol
- Creating Stock Cultures of Bacteria
- Luria-Bertani Media
- M9 Minimal Media
- Protein Overexpression and Extraction
- SDS-PAGE Protocol
- Running a Cobalt Affinity Column
- Dialysis
- Centrifugal Protein Concentration and Buffer Exchange
- Using the Stirred Cell Concentrator
Data Collection
- Gap Sampling
- Water Suppression with presaturation pulses (zgpr/zgcppr
- [[Non-uniform Sampling]
- Collecting a 15N Edited HSQC
- Collecting CEST Data
Data Processing and Analysis
- Analysis of 1D Line-Broadening Screen
- FastModelFree
- 2D NMR Analysis (CCPNMR)
- 1H NMR Analysis (SIMCA)
- 1H NMR Analysis (ACDLab)
- Processing CEST Data
- Titration Data Analysis in nmrPipe
- Non-Uniform Sampling
- [NMRFAM-SPARKY Guide]]
- NMRFAM-SPARKY: Automated Peak Assignment
- NMR Processing in Linux and Windows
Miscellaneous
- Agarose Gel
- 700 MHz NMR checklist
- 500 MHz NMR checklist
- 1D Macro
- Setting Up a Virtual Screen with AutoDock
- Simple Protein Crosslinking
- 1D NMR Titrations
Cell Culturing
Metabolomics
- MetPa for metabolomics
- Making Heatmaps
- Metabolite Extraction
- One way ANOVA in R
- P-Value adjustment for multiple comparisons
- PCA-Utils
- NMR Tube Deep Cleaning
- Noise Removal for PCA
- Weighted Linear Least Squares
- Serum Preparation for 1D NMR
- Urine Preparation for 1D NMR
Safety and Inspections
Demos
Subcategories
This category has the following 22 subcategories, out of 22 total.
A
- AutoDock (2 P)
B
- Bioscreen (4 P)
C
- Cell Culturing (16 P)
- Computer Simulation (4 P)
D
- Demos (12 P)
E
F
- FAST-NMR (6 P)
G
- Gel (2 P)
- General Maintenance (14 P)
L
- Lab Safety (14 P)
M
- Molecular Docking (1 P)
- MVAPACK (4 P)
N
- Needs Updating (25 P)
P
- Proteomics (empty)
S
- Sample Preparation (9 P)
T
- Troubleshooting (2 P)
U
- Update Wiki (3 P)