Agarose Gel

Preparing 1% Agarose Gel

1. Measure 0.6 g of agarose
2. Pour the agarose powder into microwavable flask with 60 mL of 1XTAE buffer
3. Microwave for 1- 3 min (until the agarose is completely dissolved)
4. Cool down the agarose and add ethidium bromide to a final concentration of approximately 0.2 to 0.5 ug/mL
5. Pour the agarose into a gel try with the well comb in place
6. Let sit at room temperature for about half an hour

N.B Ethidium bromide is a known mutagen.

Running Agarose Gel

1. add running buffer to the gel apparatus
2. carefully load samples in to the well
3. load marker