Category:Protocols: Difference between revisions

General Maintenance

• Changing the high pressure dewar
• Filling a Magnet with Nitrogen
• Autoclaving Laboratory Glassware and Media
• Chemical Disinfection of Glassware
• Requesting Balance Calibration
• Requesting Pipette Calibration
• Using the UV-Vis
• Using/Maintaining pH Meter

Protein Preparation

• Buffer Exchange and Solution Concentration
• Finding a Protein Target on the NESG website
• Choosing a Plasmid
• Plasmid Purification and Transformation Protocol
• Creating Stock Cultures of Bacteria
• Luria-Bertani Media
• M9 Minimal Media
• Protein Overexpression and Extraction
• SDS-PAGE Protocol
• Running a Cobalt Affinity Column
• Dialysis
• Centrifugal Protein Concentration and Buffer Exchange
• Using the Stirred Cell Concentrator

Data Collection

• Collecting a 15N Edited HSQC
• Collecting CEST Data

Data Processing and Analysis

• Analysis of 1D Line-Broadening Screen
• FastModelFree
• 2D NMR Analysis (CCPNMR)
• 1H NMR Analysis (SIMCA)
• 1H NMR Analysis (ACDLab)
• Processing CEST Data
• Titration Data Analysis in nmrPipe

Miscellaneous

• Agarose Gel
• 700 MHz NMR checklist
• 500 MHz NMR checklist
• 1D Macro
• Setting Up a Virtual Screen with AutoDock
• Simple Protein Crosslinking
• 1D NMR Titrations