Category:Protocols: Difference between revisions
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''General Maintenance'' | |||
*[[Changing the high pressure dewar]] | *[[Changing the high pressure dewar]] | ||
*[[Filling a Magnet with Nitrogen]] | *[[Filling a Magnet with Nitrogen]] | ||
*[[Autoclaving Laboratory Glassware and Media]] | *[[Autoclaving Laboratory Glassware and Media]] | ||
*[[Chemical Disinfection of Glassware]] | *[[Chemical Disinfection of Glassware]] | ||
''Protein Preparation'' | |||
*[[Finding a Protein Target on the NESG website]] | *[[Finding a Protein Target on the NESG website]] | ||
*[[Choosing a Plasmid]] | *[[Choosing a Plasmid]] | ||
| Line 15: | Line 17: | ||
*[[Centrifugal Protein Concentration and Buffer Exchange]] | *[[Centrifugal Protein Concentration and Buffer Exchange]] | ||
*[[Using the Stirred Cell Concentrator]] | *[[Using the Stirred Cell Concentrator]] | ||
''Data Collection'' | |||
*[[Collecting a 15N Edited HSQC]] | *[[Collecting a 15N Edited HSQC]] | ||
*[[Collecting CEST Data]] | |||
''Data Processing and Analysis'' | |||
*[[Processing CEST Data]] | |||
*[[Titration Data Analysis in nmrPipe]] | *[[Titration Data Analysis in nmrPipe]] | ||
''Miscellaneous'' | |||
*[[Setting Up a Virtual Screen with AutoDock]] | *[[Setting Up a Virtual Screen with AutoDock]] | ||
*[[Simple Protein Crosslinking]] | *[[Simple Protein Crosslinking]] | ||
*[[Creating a New Wiki Page]] | *[[Creating a New Wiki Page]] | ||
Revision as of 15:02, 20 March 2017
General Maintenance
- Changing the high pressure dewar
- Filling a Magnet with Nitrogen
- Autoclaving Laboratory Glassware and Media
- Chemical Disinfection of Glassware
Protein Preparation
- Finding a Protein Target on the NESG website
- Choosing a Plasmid
- Plasmid Purification and Transformation Protocol
- Creating Stock Cultures of Bacteria
- Luria-Bertani Media
- M9 Minimal Media
- Protein Overexpression and Extraction
- SDS-PAGE Protocol
- Running a Cobalt Affinity Column
- Dialysis
- Centrifugal Protein Concentration and Buffer Exchange
- Using the Stirred Cell Concentrator
Data Collection
Data Processing and Analysis
Miscellaneous
Subcategories
This category has the following 22 subcategories, out of 22 total.
A
- AutoDock (2 P)
B
- Bioscreen (4 P)
C
- Cell Culturing (16 P)
- Computer Simulation (4 P)
D
- Demos (12 P)
E
F
- FAST-NMR (6 P)
G
- Gel (2 P)
- General Maintenance (14 P)
L
- Lab Safety (14 P)
M
- Molecular Docking (1 P)
- MVAPACK (4 P)
N
- Needs Updating (25 P)
P
- Proteomics (empty)
S
- Sample Preparation (9 P)
T
- Troubleshooting (2 P)
U
- Update Wiki (3 P)