Choosing a Plasmid: Difference between revisions
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# Optimized for expression in E. coli. | # Optimized for expression in E. coli. | ||
[[category:Protein_Preparation]] | [[category:Protein_Preparation]] | ||
[[category:Cell_Culturing]] | [[category:Cell_Culturing]] | ||
Latest revision as of 20:30, 21 July 2022
Choosing a Plasmid
This step is necessary if your protein target does not come with a designated plasmid. Instead of steps, these are requirements for what should be located on your plasmid.
- Protein target sequence inserted
- N- or C-terminal histidine tag of varying length
- Antibiotic selection sequence- ampicillinm, kanamycin, or chloramphenicol.
- Optional- his tag cleavage site. Either TEV or thrombin.
- Optimized for expression in E. coli.