Collecting CEST Data: Difference between revisions
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# Click “execute” and hit “ok”. | # Click “execute” and hit “ok”. | ||
# Carrier Positions | # Carrier Positions | ||
##1H water pos. (O1) = 3290.987 Hz (this can be changed) | |||
##15N carrier pos = 118 ppm | |||
##13C carrier pos = 118ppm (pseudo dimension) | |||
# Click “Ok” | # Click “Ok” | ||
# Calibrated pulse values: | # Calibrated pulse values: | ||
##1H hard pulse = 10.25us @ -7.558748 dB (can be changed) | |||
##15N hard pulse = 35us @ -19.0309 dB | |||
##13C hard pulse = 12us @ -18.60338 dB | |||
# Click “Ok” | # Click “Ok” | ||
# Values related to the weak B1 irradiation: | # Values related to the weak B1 irradiation: | ||
##15N weak B1 field = 25 Hz (can be changed) | |||
##Upfield irradiation offset limit = 100 ppm (can be changed) | |||
##Downfield irradiation offset limit = 140 ppm (can be changed) | |||
##Offset step size = 25 Hz (can be changed) | |||
# Click “Ok” | # Click “Ok” | ||
# The experiment dataset will now be created. F1 is the pseudo dimension with the TD being the # of offsets that will be collected. The delays for these offsets can be found it FQLIST/FQ3LIST/n15cest_offset_std | # The experiment dataset will now be created. F1 is the pseudo dimension with the TD being the # of offsets that will be collected. The delays for these offsets can be found it FQLIST/FQ3LIST/n15cest_offset_std. This list name is overwritten each time a new CEST experiment is created so it is best to rename. | ||
This list name is overwritten each time a new CEST experiment is created so it is best to rename. | |||
# Edit F3 and F2 TDs. | # Edit F3 and F2 TDs. | ||
# DS must be >= 32. | # DS must be >= 32. | ||
| Line 41: | Line 40: | ||
##“xfb s23” plane 1 | ##“xfb s23” plane 1 | ||
# Also, collecting the first plane is advisable. | # Also, collecting the first plane is advisable. | ||
[[category:Data_Collection]] | |||
Latest revision as of 20:30, 21 July 2022
Collecting CEST Data
- Open TopSpin >= 3.2.X
- Edit temperature
- “edte”
- Create a dataset and collect a NHSQC to check resolution and NS
- Type “edpy”, select n15cest_gb.py.
- Click “execute” and hit “ok”.
- Carrier Positions
- 1H water pos. (O1) = 3290.987 Hz (this can be changed)
- 15N carrier pos = 118 ppm
- 13C carrier pos = 118ppm (pseudo dimension)
- Click “Ok”
- Calibrated pulse values:
- 1H hard pulse = 10.25us @ -7.558748 dB (can be changed)
- 15N hard pulse = 35us @ -19.0309 dB
- 13C hard pulse = 12us @ -18.60338 dB
- Click “Ok”
- Values related to the weak B1 irradiation:
- 15N weak B1 field = 25 Hz (can be changed)
- Upfield irradiation offset limit = 100 ppm (can be changed)
- Downfield irradiation offset limit = 140 ppm (can be changed)
- Offset step size = 25 Hz (can be changed)
- Click “Ok”
- The experiment dataset will now be created. F1 is the pseudo dimension with the TD being the # of offsets that will be collected. The delays for these offsets can be found it FQLIST/FQ3LIST/n15cest_offset_std. This list name is overwritten each time a new CEST experiment is created so it is best to rename.
- Edit F3 and F2 TDs.
- DS must be >= 32.
- NS must be >= 2.
- Edit routing.
- Click “Edit” for CPDPRG, change 90x240y90x to 90x_240y_90x
- Make sure P50, P51, and P52 are all 500us
- Calculate receiver gain
- “rga”
- Edit D2 (t1 time)
- Must also edit D3 so that D3 > D2.
- Check ZGOPTNS
- “zg”
- The planes can be checked with “XFB”
- “xfb s23” plane 1
- Also, collecting the first plane is advisable.