Dialysis: Difference between revisions
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(Created page with "Dialysis # Grab enough dialysis tubing (or a Float-a-lyzer) to hold your sample. If using tubing, make sure there is extra tubing to fold over and clip. # Fold one end of the...") |
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# Replace the buffer after 4 hours and 2 more times over the next 16-24 hours. | # Replace the buffer after 4 hours and 2 more times over the next 16-24 hours. | ||
# The total amount of buffer that the sample is exposed to should be 100x the sample volume. | # The total amount of buffer that the sample is exposed to should be 100x the sample volume. | ||
##Example: 10mL sample dialyzed against 1L of new buffer. | |||
[[category:Protein_Preparation]] | |||
Latest revision as of 20:25, 21 July 2022
Dialysis
- Grab enough dialysis tubing (or a Float-a-lyzer) to hold your sample. If using tubing, make sure there is extra tubing to fold over and clip.
- Fold one end of the tubing two times and clip it with an alligator clip.
- Optional: Add a styrofoam ring around the tubing to help it float later.
- Insert your sample into the tube.
- Fold the other end of the tubing twice and clip with a second alligator clip.
- Place the sample and tubing into a large beaker. Add enough of the new buffer to completely cover the sample or to make it float without touching the bottom of the beaker.
- Place the beaker into a refrigerator on a stir plate and stir slowly.
- Replace the buffer after 4 hours and 2 more times over the next 16-24 hours.
- The total amount of buffer that the sample is exposed to should be 100x the sample volume.
- Example: 10mL sample dialyzed against 1L of new buffer.