Choosing a Plasmid: Difference between revisions
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(Created page with "Choosing a Plasmid This step is necessary if your protein target does not come with a designated plasmid. Instead of steps, these are requirements for what should be located ...") |
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# Optional- his tag cleavage site. Either TEV or thrombin. | # Optional- his tag cleavage site. Either TEV or thrombin. | ||
# Optimized for expression in E. coli. | # Optimized for expression in E. coli. | ||
[[category:Protocols|Protocols]] | |||
[[category:Protein_Preparation]] | |||
Revision as of 03:06, 11 September 2021
Choosing a Plasmid
This step is necessary if your protein target does not come with a designated plasmid. Instead of steps, these are requirements for what should be located on your plasmid.
- Protein target sequence inserted
- N- or C-terminal histidine tag of varying length
- Antibiotic selection sequence- ampicillinm, kanamycin, or chloramphenicol.
- Optional- his tag cleavage site. Either TEV or thrombin.
- Optimized for expression in E. coli.