UV-Vis tutorial

See also Using the UV-Vis

UV-Vis tutorial

1. Turn on all hardware, Computer, UV-Vis and Printer. The switch for UV is on the back left side, feel around for it you’ll find it. The printer is on the front right side, again feel around you’ll find it.
2. After the CPU is done warming up there will be a DOS prompt “C:/” and it might be flashing. TYPE UV and PRESS ENTER.
3. After you press enter, a blue screen will pop up and have a list of things you can do. I have been using GENERAL SCAN, the first thing on the list. PRESS ENTER to start
4. Once you are working in the GENERAL SCAN software, you want to use the F_ keys, the program calls them softkeys but I call them F_ keys.
5. Press F4 to set up your acquisition parameters and use the U/D arrows keys to highlight the parameter you want to change and hit ENTER. I have only changed the scanning range (274-284nm for HSA) and have left everything else alone. When finished use ESC key to exit
6. To start a run place blank in cuvette holder, shut lid and hit the F2 key (measure blank). This will measure your blank sample and bring you to a new “window” with your spectrum displayed and new F_key possibilities. It is VERY IMPORTANT to note that this instrument does not save your blank. Make sure after each blank follow #7&8
7. To rescale your spectrum hit F3. This will walk you through the X and Y ranges you want to look at.
8. After you have rescaled mark interesting peaks by hitting F2 (cursor). Use L/R arrow keys to place the little arrow on the peak you want then hit F1 (mark).
9. To exit the cursor mode hit F10. This will only bring you back to the measure blank “window” from step 6.
10. Place sample in instrument and hit F1 to measure sample. You can follow steps 8-9 again to find interesting peaks
11. To print, hit F9 (hardcopy) while you are still in the measure window.
12. Hit F10 to exit back to main window and then exit program when finished