Using the Stirred Cell Concentrator - Revision history

Mjeppesen at 16:55, 14 January 2022

2022-01-14T16:55:56Z

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	# Disassemble and clean.		# Disassemble and clean.

	[[category:~~Protocols~~\|Stirred Cell Concentrator]]		[[category:Needs_Updating\|Stirred Cell Concentrator]] <!-- pictures would be nice for this-->
	[[category:Protein_Preparation\|Stirred Cell Concentrator]]		[[category:Protein_Preparation\|Stirred Cell Concentrator]]
	[[category:General_Maintenance\|Stirred Cell Concentrator]]		[[category:General_Maintenance\|Stirred Cell Concentrator]]

Mjeppesen: LearningSorting

2021-09-11T05:15:34Z

LearningSorting

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	# Disassemble and clean.		# Disassemble and clean.

	[[category:Protocols]]		[[category:Protocols\|Stirred Cell Concentrator]]
	[[category:Protein_Preparation]]		[[category:Protein_Preparation\|Stirred Cell Concentrator]]
	[[category:General_Maintenance]]		[[category:General_Maintenance\|Stirred Cell Concentrator]]

Mjeppesen: LearningSorting

2021-09-11T04:58:28Z

LearningSorting

← Older revision		Revision as of 04:58, 11 September 2021
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	# Disassemble and clean.		# Disassemble and clean.

	[[category:~~Protocols\|~~Protocols]]		[[category:Protocols]]
	[[category:Protein_Preparation]]		[[category:Protein_Preparation]]
	[[category:General_Maintenance]]		[[category:General_Maintenance]]

Mjeppesen: Added to Category

2021-09-11T03:14:53Z

Added to Category

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	# Turn the pressure release valve to the horizontal position to relieve pressure.		# Turn the pressure release valve to the horizontal position to relieve pressure.
	# Disassemble and clean.		# Disassemble and clean.

			[[category:Protocols\|Protocols]]
			[[category:Protein_Preparation]]
			[[category:General_Maintenance]]

Jcatazaro: Created page with "Using the Stirred Cell Concentrator # Obtain a 44 mm membrane for the concentrator. Handle the membrane by touching only its edges. # Place the membrane, shiny side down, in..."

2017-03-14T21:03:21Z

Created page with "Using the Stirred Cell Concentrator # Obtain a 44 mm membrane for the concentrator. Handle the membrane by touching only its edges. # Place the membrane, shiny side down, in..."

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Using the Stirred Cell Concentrator

# Obtain a 44 mm membrane for the concentrator. Handle the membrane by touching only its edges.
# Place the membrane, shiny side down, in a beaker of nanopure water (100 mL).
# Change the water in the beaker three times over the next hour. This ensures that the storage buffer is completely removed from the membrane before use.
# Collect all of the parts needed to assemble the concentrator. Consult the manual if necessary.
# Lubricate the two red O-rings adequately. You should be able to slide your gloved fingers easily around them.
# When the membrane is clean, place it shiny side up on the grooved bottom piece of the concentrator.
# Place a red O-ring on the top of the membrane. Be sure to not get any lubricant all over the shiny surface.
# Place the cell body over the grooved piece and membrane and screw on the bottom plate. Ensure that the cell is sealed but do not over tighten.
# Place the stir bar insert into the concentrator cell.
# Insert the sample that is to be concentrated into the cell. Do not exceed the 50 mL mark.
# Slide the top of the concentrator onto the cell. It should go on easily. Forcing the lid on will dislodge the O-ring. Apply more lubricant to the O-ring if necessary.
# Turn the pressure release valve (black lever) on the cap to the horizontal position.
# Screw the gas inlet onto the cap. Do not over tighten.
# Place the cell into the retaining stand and turn the pressure release valve to the vertical position. Place the cell and stand on a stir plate.
# Turn on the air pressure slowly.
# Set the regulator to 50-55 psi. Do not let the pressure exceed 75 psi.
# Once the cell is pressurized, start stirring. The vortex should go down 1/3 of the total volume of the liquid.
# Concentrate to a volume greater than 2.5 mL.
# Turn off the air pressure.
# Turn the pressure release valve to the horizontal position to relieve pressure.
# Disassemble and clean.