Dialysis - Revision history

Wiki Administrator at 20:25, 21 July 2022

2022-07-21T20:25:35Z

← Older revision		Revision as of 20:25, 21 July 2022
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	##Example: 10mL sample dialyzed against 1L of new buffer.		##Example: 10mL sample dialyzed against 1L of new buffer.

	~~[[category:Protocols]]~~
	[[category:Protein_Preparation]]		[[category:Protein_Preparation]]

Mjeppesen: LearningSorting

2021-09-11T04:52:54Z

LearningSorting

← Older revision		Revision as of 04:52, 11 September 2021
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	##Example: 10mL sample dialyzed against 1L of new buffer.		##Example: 10mL sample dialyzed against 1L of new buffer.

	[[category:~~Protocols\|~~Protocols]]		[[category:Protocols]]
	[[category:Protein_Preparation]]		[[category:Protein_Preparation]]

Mjeppesen: Added to Category

2021-09-11T03:12:45Z

Added to Category

← Older revision		Revision as of 03:12, 11 September 2021
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	# The total amount of buffer that the sample is exposed to should be 100x the sample volume.		# The total amount of buffer that the sample is exposed to should be 100x the sample volume.
	##Example: 10mL sample dialyzed against 1L of new buffer.		##Example: 10mL sample dialyzed against 1L of new buffer.

			[[category:Protocols\|Protocols]]
			[[category:Protein_Preparation]]

Jcatazaro at 20:58, 14 March 2017

2017-03-14T20:58:35Z

← Older revision		Revision as of 20:58, 14 March 2017
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	# Replace the buffer after 4 hours and 2 more times over the next 16-24 hours.		# Replace the buffer after 4 hours and 2 more times over the next 16-24 hours.
	# The total amount of buffer that the sample is exposed to should be 100x the sample volume.		# The total amount of buffer that the sample is exposed to should be 100x the sample volume.
	Example: 10mL sample dialyzed against 1L of new buffer.		##Example: 10mL sample dialyzed against 1L of new buffer.

Jcatazaro: Created page with "Dialysis # Grab enough dialysis tubing (or a Float-a-lyzer) to hold your sample. If using tubing, make sure there is extra tubing to fold over and clip. # Fold one end of the..."

2017-03-14T20:58:19Z

Created page with "Dialysis # Grab enough dialysis tubing (or a Float-a-lyzer) to hold your sample. If using tubing, make sure there is extra tubing to fold over and clip. # Fold one end of the..."

New page

Dialysis

# Grab enough dialysis tubing (or a Float-a-lyzer) to hold your sample. If using tubing, make sure there is extra tubing to fold over and clip.
# Fold one end of the tubing two times and clip it with an alligator clip.
##Optional: Add a styrofoam ring around the tubing to help it float later.
# Insert your sample into the tube.
# Fold the other end of the tubing twice and clip with a second alligator clip.
# Place the sample and tubing into a large beaker. Add enough of the new buffer to completely cover the sample or to make it float without touching the bottom of the beaker.
# Place the beaker into a refrigerator on a stir plate and stir slowly.
# Replace the buffer after 4 hours and 2 more times over the next 16-24 hours.
# The total amount of buffer that the sample is exposed to should be 100x the sample volume.
Example: 10mL sample dialyzed against 1L of new buffer.