Collecting CEST Data - Revision history

Mjeppesen at 20:30, 21 July 2022

2022-07-21T20:30:54Z

← Older revision		Revision as of 20:30, 21 July 2022
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	# Also, collecting the first plane is advisable.		# Also, collecting the first plane is advisable.

	~~[[category:Protocols]]~~
	[[category:Data_Collection]]		[[category:Data_Collection]]

Mjeppesen: LearningSorting

2021-09-11T04:57:11Z

LearningSorting

← Older revision		Revision as of 04:57, 11 September 2021
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	# Also, collecting the first plane is advisable.		# Also, collecting the first plane is advisable.

	[[category:~~Protocols\|~~Protocols]]		[[category:Protocols]]
	[[category:Data_Collection]]		[[category:Data_Collection]]

Mjeppesen: Added to Category

2021-09-11T03:18:59Z

Added to Category

← Older revision		Revision as of 03:18, 11 September 2021
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	##“xfb s23” plane 1		##“xfb s23” plane 1
	# Also, collecting the first plane is advisable.		# Also, collecting the first plane is advisable.

			[[category:Protocols\|Protocols]]
			[[category:Data_Collection]]

Jcatazaro at 21:29, 14 March 2017

2017-03-14T21:29:44Z

← Older revision		Revision as of 21:29, 14 March 2017
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	# Click “execute” and hit “ok”.		# Click “execute” and hit “ok”.
	# Carrier Positions		# Carrier Positions
	**1H water pos. (O1) = 3290.987 Hz (this can be changed)		##1H water pos. (O1) = 3290.987 Hz (this can be changed)
	**15N carrier pos = 118 ppm		##15N carrier pos = 118 ppm
	**13C carrier pos = 118ppm (pseudo dimension)		##13C carrier pos = 118ppm (pseudo dimension)
	# Click “Ok”		# Click “Ok”
	# Calibrated pulse values:		# Calibrated pulse values:
	**1H hard pulse = 10.25us @ -7.558748 dB (can be changed)		##1H hard pulse = 10.25us @ -7.558748 dB (can be changed)
	**15N hard pulse = 35us @ -19.0309 dB		##15N hard pulse = 35us @ -19.0309 dB
	**13C hard pulse = 12us @ -18.60338 dB		##13C hard pulse = 12us @ -18.60338 dB
	# Click “Ok”		# Click “Ok”
	# Values related to the weak B1 irradiation:		# Values related to the weak B1 irradiation:
	*15N weak B1 field = 25 Hz (can be changed)		##15N weak B1 field = 25 Hz (can be changed)
	*Upfield irradiation offset limit = 100 ppm (can be changed)		##Upfield irradiation offset limit = 100 ppm (can be changed)
	*Downfield irradiation offset limit = 140 ppm (can be changed)		##Downfield irradiation offset limit = 140 ppm (can be changed)
	*Offset step size = 25 Hz (can be changed)		##Offset step size = 25 Hz (can be changed)
	# Click “Ok”		# Click “Ok”
	# The experiment dataset will now be created. F1 is the pseudo dimension with the TD being the # of offsets that will be collected. The delays for these offsets can be found it FQLIST/FQ3LIST/n15cest_offset_std. This list name is overwritten each time a new CEST experiment is created so it is best to rename.		# The experiment dataset will now be created. F1 is the pseudo dimension with the TD being the # of offsets that will be collected. The delays for these offsets can be found it FQLIST/FQ3LIST/n15cest_offset_std. This list name is overwritten each time a new CEST experiment is created so it is best to rename.

Jcatazaro at 21:29, 14 March 2017

2017-03-14T21:29:00Z

← Older revision		Revision as of 21:29, 14 March 2017
Line 9:		Line 9:
	# Click “execute” and hit “ok”.		# Click “execute” and hit “ok”.
	# Carrier Positions		# Carrier Positions
	*1H water pos. (O1) = 3290.987 Hz (this can be changed)		**1H water pos. (O1) = 3290.987 Hz (this can be changed)
	*15N carrier pos = 118 ppm		**15N carrier pos = 118 ppm
	*13C carrier pos = 118ppm (pseudo dimension)		**13C carrier pos = 118ppm (pseudo dimension)
	# Click “Ok”		# Click “Ok”
	# Calibrated pulse values:		# Calibrated pulse values:
	*1H hard pulse = 10.25us @ -7.558748 dB (can be changed)		**1H hard pulse = 10.25us @ -7.558748 dB (can be changed)
	*15N hard pulse = 35us @ -19.0309 dB		**15N hard pulse = 35us @ -19.0309 dB
	*13C hard pulse = 12us @ -18.60338 dB		**13C hard pulse = 12us @ -18.60338 dB
	# Click “Ok”		# Click “Ok”
	# Values related to the weak B1 irradiation:		# Values related to the weak B1 irradiation:

Jcatazaro at 21:28, 14 March 2017

2017-03-14T21:28:32Z

← Older revision		Revision as of 21:28, 14 March 2017
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	*Offset step size = 25 Hz (can be changed)		*Offset step size = 25 Hz (can be changed)
	# Click “Ok”		# Click “Ok”
	# The experiment dataset will now be created. F1 is the pseudo dimension with the TD being the # of offsets that will be collected. The delays for these offsets can be found it FQLIST/FQ3LIST/n15cest_offset_std		# The experiment dataset will now be created. F1 is the pseudo dimension with the TD being the # of offsets that will be collected. The delays for these offsets can be found it FQLIST/FQ3LIST/n15cest_offset_std. This list name is overwritten each time a new CEST experiment is created so it is best to rename.
	This list name is overwritten each time a new CEST experiment is created so it is best to rename.
	# Edit F3 and F2 TDs.		# Edit F3 and F2 TDs.
	# DS must be >= 32.		# DS must be >= 32.

Jcatazaro: Created page with "Collecting CEST Data # Open TopSpin >= 3.2.X # Edit temperature ##“edte” # Create a dataset and collect a NHSQC to check resolution and NS # Type “edpy”, select n15c..."

2017-03-14T21:28:02Z

Created page with "Collecting CEST Data # Open TopSpin >= 3.2.X # Edit temperature ##“edte” # Create a dataset and collect a NHSQC to check resolution and NS # Type “edpy”, select n15c..."

New page

Collecting CEST Data

# Open TopSpin >= 3.2.X
# Edit temperature
##“edte”
# Create a dataset and collect a NHSQC to check resolution and NS
# Type “edpy”, select n15cest_gb.py.
# Click “execute” and hit “ok”.
# Carrier Positions
*1H water pos. (O1) = 3290.987 Hz (this can be changed)
*15N carrier pos = 118 ppm
*13C carrier pos = 118ppm (pseudo dimension)
# Click “Ok”
# Calibrated pulse values:
*1H hard pulse = 10.25us @ -7.558748 dB (can be changed)
*15N hard pulse = 35us @ -19.0309 dB
*13C hard pulse = 12us @ -18.60338 dB
# Click “Ok”
# Values related to the weak B1 irradiation:
*15N weak B1 field = 25 Hz (can be changed)
*Upfield irradiation offset limit = 100 ppm (can be changed)
*Downfield irradiation offset limit = 140 ppm (can be changed)
*Offset step size = 25 Hz (can be changed)
# Click “Ok”
# The experiment dataset will now be created. F1 is the pseudo dimension with the TD being the # of offsets that will be collected. The delays for these offsets can be found it FQLIST/FQ3LIST/n15cest_offset_std
This list name is overwritten each time a new CEST experiment is created so it is best to rename.
# Edit F3 and F2 TDs.
# DS must be >= 32.
# NS must be >= 2.
# Edit routing.
# Click “Edit” for CPDPRG, change 90x240y90x to 90x_240y_90x
# Make sure P50, P51, and P52 are all 500us
# Calculate receiver gain
##“rga”
# Edit D2 (t1 time)
# Must also edit D3 so that D3 > D2.
# Check ZGOPTNS
# “zg”
# The planes can be checked with “XFB”
##“xfb s23” plane 1
# Also, collecting the first plane is advisable.