500 MHz NMR checklist - Revision history

Mjeppesen at 06:19, 20 January 2022

2022-01-20T06:19:26Z

← Older revision		Revision as of 06:19, 20 January 2022
Line 76:		Line 76:
	2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.		2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.

	~~[[category:Protocols\|NMR checklist 500]]~~
	[[category:NMR_Usage\|NMR checklist 500]]		[[category:NMR_Usage\|NMR checklist 500]]

Mjeppesen: LearningSorting

2021-09-11T04:26:14Z

LearningSorting

← Older revision		Revision as of 04:26, 11 September 2021
Line 76:		Line 76:
	2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.		2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.

	[[category:Protocols\|~~Protocols~~]]		[[category:Protocols\|NMR checklist 500]]
	[[category:NMR_Usage]]		[[category:NMR_Usage\|NMR checklist 500]]

Mjeppesen: Added to Category

2021-09-11T03:32:50Z

Added to Category

← Older revision		Revision as of 03:32, 11 September 2021
Line 75:		Line 75:

	2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.		2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.

			[[category:Protocols\|Protocols]]
			[[category:NMR_Usage]]

Jcatazaro at 15:31, 20 March 2017

2017-03-20T15:31:46Z

← Older revision		Revision as of 15:31, 20 March 2017
Line 75:		Line 75:

	2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.		2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.

	~~[[Category:Protocols]]~~
	~~[[Category:Metabolomics]]~~

Bozhang at 05:03, 13 October 2012

2012-10-13T05:03:09Z

← Older revision		Revision as of 05:03, 13 October 2012
Line 77:		Line 77:

	[[Category:Protocols]]		[[Category:Protocols]]
	~~[[Category:Lab safety and management]]~~
	[[Category:Metabolomics]]		[[Category:Metabolomics]]

Bozhang at 03:46, 13 October 2012

2012-10-13T03:46:27Z

← Older revision		Revision as of 03:46, 13 October 2012
Line 1:		Line 1:
	~~==CHECKLIST _500 MHz NMR facility manipulation==~~

	===Check in===		===Check in===
	Make sure the system is accessible, e.g. not being used or under maintenance		Make sure the system is accessible, e.g. not being used or under maintenance

Bozhang at 00:15, 22 September 2012

2012-09-22T00:15:36Z

← Older revision		Revision as of 00:15, 22 September 2012
Line 80:		Line 80:
	[[Category:Protocols]]		[[Category:Protocols]]
	[[Category:Lab safety and management]]		[[Category:Lab safety and management]]
			[[Category:Metabolomics]]

Wiki Administrator: 5 revisions: Lab safety and management

2012-03-13T00:00:09Z

5 revisions: Lab safety and management

← Older revision	Revision as of 00:00, 13 March 2012
(No difference)

Bozhang at 20:39, 12 September 2011

2011-09-12T20:39:50Z

← Older revision		Revision as of 20:39, 12 September 2011
Line 8:		Line 8:
	Check your belongings on your body and remove anything with ferromagnetic properties such as electronic devices and keep them away from the probe		Check your belongings on your body and remove anything with ferromagnetic properties such as electronic devices and keep them away from the probe

	Login Load your sample(s)		Login

			Load your sample(s)

	Start topspin software		Start topspin software
Line 20:		Line 22:
	Type in “gradshim” (use the proper gradshim settings for a heavily protonated or deuterated solvent)		Type in “gradshim” (use the proper gradshim settings for a heavily protonated or deuterated solvent)

	Wait for it to finish and evaluate the results, if they are not good repeat gradshim Close gradshim		Wait for it to finish and evaluate the results, if they are not good repeat gradshim

			Close gradshim

	Make sure the lock light is on Type in “atma” to tune and match the probe Find the proton 90 time (“p1”)		Make sure the lock light is on Type in “atma” to tune and match the probe Find the proton 90 time (“p1”)
Line 47:		Line 51:

	===During the running===		===During the running===

	Pay attention to the weather conditions especially any electric outage. Follow the “cryo platform outage procedure” as being described by Joe.		Pay attention to the weather conditions especially any electric outage. Follow the “cryo platform outage procedure” as being described by Joe.

	===Check out/end of run===		===Check out/end of run===

	If any samples are reloaded into the belt, verify that the sample tube is not set to the wrong depth (spinner tightness)		If any samples are reloaded into the belt, verify that the sample tube is not set to the wrong depth (spinner tightness)

	Load the reference CDCl3 sample		Load the reference CDCl3 sample

Line 59:		Line 66:
	Eject the sample and remove the NMR tube from the spinner Leave the spinner in the black holder at the workstation		Eject the sample and remove the NMR tube from the spinner Leave the spinner in the black holder at the workstation

	Sign up the log book and clear up the area If you use the BACS automation: First thing is to: Stop the running (when all samples are finished)		Sign up the log book and clear up the area

			If you use the BACS automation: First thing is to: Stop the running (when all samples are finished)

	Type in “edprosol” and change p1 value back (same procedure above)		Type in “edprosol” and change p1 value back (same procedure above)

	===Note===		===Note===

	1. Make sure your tube is clean, the spinner is clean, the sample is 600 µL and the tube is in the right depth of the spinner, holding tightly. If you have multiple samples, they need to have the same volume and fit into the spinners at the same height.		1. Make sure your tube is clean, the spinner is clean, the sample is 600 µL and the tube is in the right depth of the spinner, holding tightly. If you have multiple samples, they need to have the same volume and fit into the spinners at the same height.

Bozhang at 22:16, 4 August 2011

2011-08-04T22:16:03Z

← Older revision		Revision as of 22:16, 4 August 2011
Line 68:		Line 68:
	2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.		2. Use a container or a rack to carry the NMR tubes from your own lab to the NMR lab. Make sure the sample is not reactive with the solvents and uniformly dissolved in your solvent.

			[[Category:Protocols]]
	[[Category:Lab safety and management]]		[[Category:Lab safety and management]]