Using the UV-Vis

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Using the UV-Vis

  1. At least 10 minutes prior to use, turn on UV-Vis.
  2. Use keypad to select single wavelength.
  3. Specify which wavelength you will run.
    1. 600 for cell cultures.
    2. 280 protein purification.
    3. 295 for Bradford test.
  4. Insert blank cuvette and press 0A/100%T button.
    1. Plastic cuvette or quartz cuvette.
  5. Insert sample cuvette and press play button.
  6. Record absorbance.
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