Dialysis

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Dialysis

  1. Grab enough dialysis tubing (or a Float-a-lyzer) to hold your sample. If using tubing, make sure there is extra tubing to fold over and clip.
  2. Fold one end of the tubing two times and clip it with an alligator clip.
    1. Optional: Add a styrofoam ring around the tubing to help it float later.
  3. Insert your sample into the tube.
  4. Fold the other end of the tubing twice and clip with a second alligator clip.
  5. Place the sample and tubing into a large beaker. Add enough of the new buffer to completely cover the sample or to make it float without touching the bottom of the beaker.
  6. Place the beaker into a refrigerator on a stir plate and stir slowly.
  7. Replace the buffer after 4 hours and 2 more times over the next 16-24 hours.
  8. The total amount of buffer that the sample is exposed to should be 100x the sample volume.
    1. Example: 10mL sample dialyzed against 1L of new buffer.
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