Agarose Gel

From BioNMR
Jump to: navigation, search


Preparing 1% Agarose Gel

  1. Measure 0.6 g of agarose
  2. Pour the agarose powder into microwavable flask with 60 mL of 1XTAE buffer
  3. Microwave for 1- 3 min (until the agarose is completely dissolved)
  4. Cool down the agarose and add ethidium bromide to a final concentration of approximately 0.2 to 0.5 ug/mL
  5. Pour the agarose into a gel try with the well comb in place
  6. Let sit at room temperature for about half an hour

N.B Ethidium bromide is a known mutagen.

Running Agarose Gel

  1. add running buffer to the gel apparatus
  2. carefully load samples in to the well
  3. load marker
Personal tools
Namespaces
Variants
Actions
Navigation
Toolbox