User contributions for Jcatazaro

15:4415:44, 20 March 2017 diff hist −23‎ SO-FASTprocess ‎No edit summary
15:4415:44, 20 March 2017 diff hist −24‎ Running the CLEANEX Experiment ‎No edit summary current
15:4315:43, 20 March 2017 diff hist −46‎ Running CPASS ‎No edit summary
15:4315:43, 20 March 2017 diff hist −46‎ Preparing Protein for FAST-NMR ‎No edit summary
15:4315:43, 20 March 2017 diff hist −46‎ Preparing FAST-NMR Mixtures ‎No edit summary
15:4015:40, 20 March 2017 diff hist −46‎ FAST-NMR ‎No edit summary
15:4015:40, 20 March 2017 diff hist −46‎ Docking the Hits in FAST-NMR ‎No edit summary current
15:4015:40, 20 March 2017 diff hist −55‎ DOCK ‎No edit summary current
15:4015:40, 20 March 2017 diff hist −54‎ Buffer Exchange and Solution Concentration ‎No edit summary
15:3915:39, 20 March 2017 diff hist −45‎ Analysis of 1D Line-Broadening Screen ‎No edit summary
15:3915:39, 20 March 2017 diff hist −54‎ Agarose Gel ‎No edit summary
15:3915:39, 20 March 2017 diff hist +108‎ Category:Protocols ‎No edit summary
15:3715:37, 20 March 2017 diff hist −22‎ FastModelFree ‎No edit summary
15:3615:36, 20 March 2017 diff hist +19‎ Category:Protocols ‎No edit summary
15:3515:35, 20 March 2017 diff hist −48‎ Bioinformatics Tools For Annotating Proteins ‎No edit summary
15:3515:35, 20 March 2017 diff hist −55‎ AutoDockFilter ‎No edit summary
15:3415:34, 20 March 2017 diff hist −55‎ AutoDock ‎No edit summary
15:3415:34, 20 March 2017 diff hist −46‎ Annotating the Protein ‎No edit summary current
15:3315:33, 20 March 2017 diff hist −46‎ Adding Compounds To Library ‎No edit summary current
15:3215:32, 20 March 2017 diff hist −50‎ 700 MHz NMR checklist ‎No edit summary
15:3115:31, 20 March 2017 diff hist −50‎ 500 MHz NMR checklist ‎No edit summary
15:3115:31, 20 March 2017 diff hist −51‎ 2D NMR Analysis (CCPNMR) ‎No edit summary
15:3015:30, 20 March 2017 diff hist −48‎ 1H NMR Analysis (SIMCA) ‎No edit summary
15:3015:30, 20 March 2017 diff hist −48‎ 1H NMR Analysis (ACDLab) ‎No edit summary
15:2915:29, 20 March 2017 diff hist +113‎ Category:Protocols ‎No edit summary
15:2715:27, 20 March 2017 diff hist −1‎ Category:Protocols ‎No edit summary
15:2615:26, 20 March 2017 diff hist +31‎ Category:Protocols ‎No edit summary
15:2315:23, 20 March 2017 diff hist −46‎ 2D 15N-HSQC Screens For FAST-NMR ‎No edit summary
15:1915:19, 20 March 2017 diff hist −24‎ 1D NMR Titrations ‎No edit summary
15:1915:19, 20 March 2017 diff hist +23‎ Category:Protocols ‎No edit summary
15:1515:15, 20 March 2017 diff hist −23‎ 1D Macro ‎No edit summary
15:1415:14, 20 March 2017 diff hist −160‎ 1D Line Broadening Screen For FAST-NMR ‎ Blanked the page
15:1415:14, 20 March 2017 diff hist +14‎ Category:Protocols ‎No edit summary
15:1215:12, 20 March 2017 diff hist −22‎ 1D Macro ‎No edit summary
15:1115:11, 20 March 2017 diff hist −10‎ Category:Protocols ‎No edit summary
15:1015:10, 20 March 2017 diff hist +10‎ Category:Protocols ‎No edit summary
15:0215:02, 20 March 2017 diff hist −30‎ Category:Protocols ‎No edit summary
15:0215:02, 20 March 2017 diff hist +119‎ Category:Protocols ‎No edit summary

00:0600:06, 15 March 2017 diff hist −20‎ Category:Protocols ‎No edit summary
00:0500:05, 15 March 2017 diff hist +20‎ Category:Protocols ‎No edit summary
00:0300:03, 15 March 2017 diff hist +30‎ Category:Protocols ‎No edit summary

22:5322:53, 14 March 2017 diff hist −24‎ Changing the high pressure dewar ‎No edit summary
22:5222:52, 14 March 2017 diff hist +24‎ Changing the high pressure dewar ‎No edit summary
21:5021:50, 14 March 2017 diff hist +375‎ FastModelFree ‎No edit summary
21:4421:44, 14 March 2017 diff hist +97‎ FastModelFree ‎No edit summary
21:3821:38, 14 March 2017 diff hist −21‎ Processing CEST Data ‎No edit summary
21:3721:37, 14 March 2017 diff hist +1,290‎ N Processing CEST Data ‎ Created page with "Processing CEST Data # Install X11, nmrPipe, Python >=2.7, and ChemEx (from the Kay lab) # Open a c-shell with “csh” and navigate to the directory with your ser file # O..."
21:3221:32, 14 March 2017 diff hist +26‎ m Category:Protocols ‎No edit summary
21:2921:29, 14 March 2017 diff hist +4‎ Collecting CEST Data ‎No edit summary
21:2921:29, 14 March 2017 diff hist +6‎ Collecting CEST Data ‎No edit summary
21:2821:28, 14 March 2017 diff hist +1‎ Collecting CEST Data ‎No edit summary
21:2821:28, 14 March 2017 diff hist +1,572‎ N Collecting CEST Data ‎ Created page with "Collecting CEST Data # Open TopSpin >= 3.2.X # Edit temperature ##“edte” # Create a dataset and collect a NHSQC to check resolution and NS # Type “edpy”, select n15c..."
21:2521:25, 14 March 2017 diff hist +26‎ Category:Protocols ‎No edit summary
21:2421:24, 14 March 2017 diff hist +778‎ N Simple Protein Crosslinking ‎ Created page with "Simple Protein Crosslinking # Have tagged, purified protein ready before starting the rest of this procedure. # Grow up a culture of target cells (500mL is fine). # Spin dow..."
21:2321:23, 14 March 2017 diff hist +33‎ Category:Protocols ‎No edit summary
21:2321:23, 14 March 2017 diff hist +1,295‎ N Setting Up a Virtual Screen with AutoDock ‎ Created page with "Setting Up a Virtual Screen with AutoDock # Install MGLTools and AutoDock, download raccoon.py # Navigate to the MGLTools directory ## "cd /Library/MGLTools/1.5.6/bin" # Ope..." current
21:2121:21, 14 March 2017 diff hist +47‎ Category:Protocols ‎No edit summary
21:1921:19, 14 March 2017 diff hist +76‎ Titration Data Analysis in nmrPipe ‎No edit summary
21:1821:18, 14 March 2017 diff hist +1,083‎ N Titration Data Analysis in nmrPipe ‎ Created page with "Titration Data Analysis in nmrPipe 1. Type “csh” in your terminal to get a c-shell 2. In a directory that contains the data folders create an “in.list” file which ..."
21:1721:17, 14 March 2017 diff hist +40‎ Category:Protocols ‎No edit summary
21:1621:16, 14 March 2017 diff hist +1,476‎ N Collecting a 15N Edited HSQC ‎ Created page with "Collecting a Superb 15N edited HSQC (NHSQC) # Concentrate your protein as much as possible. It should be at least 50uM for 2D work and around 1mM for 3D work. # Spike in 10% ..."
21:1121:11, 14 March 2017 diff hist +34‎ Category:Protocols ‎No edit summary
21:0321:03, 14 March 2017 diff hist +1,945‎ N Using the Stirred Cell Concentrator ‎ Created page with "Using the Stirred Cell Concentrator # Obtain a 44 mm membrane for the concentrator. Handle the membrane by touching only its edges. # Place the membrane, shiny side down, in..."
21:0121:01, 14 March 2017 diff hist +41‎ Category:Protocols ‎No edit summary
21:0021:00, 14 March 2017 diff hist +940‎ N Centrifugal Protein Concentration and Buffer Exchange ‎ Created page with "Centrifugal Protein Concentration/Buffer Exchange # Obtain a centrifugal filter (Amicon or Centricon) with a molecular weight cutoff at least half of your protein’s weight...."
20:5920:59, 14 March 2017 diff hist +59‎ Category:Protocols ‎No edit summary
20:5820:58, 14 March 2017 diff hist +1‎ Dialysis ‎No edit summary
20:5820:58, 14 March 2017 diff hist +895‎ N Dialysis ‎ Created page with "Dialysis # Grab enough dialysis tubing (or a Float-a-lyzer) to hold your sample. If using tubing, make sure there is extra tubing to fold over and clip. # Fold one end of the..."
20:5720:57, 14 March 2017 diff hist +14‎ Category:Protocols ‎No edit summary
20:5620:56, 14 March 2017 diff hist +1,301‎ N Running a Cobalt Affinity Column ‎ Created page with "Running a Cobalt Affinity Column (Purifying a His-tagged Protein) # Obtain the cobalt resin from the refrigerator and a clean column with a valve and top. # Swirl the cobalt..."
20:5520:55, 14 March 2017 diff hist +38‎ Category:Protocols ‎No edit summary
20:5320:53, 14 March 2017 diff hist +23‎ Category:Protocols ‎No edit summary
20:2320:23, 14 March 2017 diff hist −3‎ Protein Overexpression and Extraction ‎No edit summary
20:2220:22, 14 March 2017 diff hist 0‎ Protein Overexpression and Extraction ‎No edit summary
20:2120:21, 14 March 2017 diff hist +6‎ Protein Overexpression and Extraction ‎No edit summary
20:1920:19, 14 March 2017 diff hist +1‎ Protein Overexpression and Extraction ‎No edit summary
20:1820:18, 14 March 2017 diff hist +1,796‎ N Protein Overexpression and Extraction ‎ Created page with "Protein Overexpression and Extraction # Prepare and sterilize a minimal media where the carbon and nitrogen source can be controlled (M9 media). Make sure the volume of the m..."
20:1320:13, 14 March 2017 diff hist +43‎ Category:Protocols ‎No edit summary
20:1020:10, 14 March 2017 diff hist +423‎ N M9 Minimal Media ‎ Created page with "M9 Minimal Media Per liter of sterile water: *5X M9 salts* 200 mL *1M MgSO4 2 mL *20% glucose** 20 mL *1M CaCl2 0.1 mL 5X M9 salts Per liter: *Na2HPO4 64 grams *KH..."
20:0920:09, 14 March 2017 diff hist +22‎ Category:Protocols ‎No edit summary
20:0820:08, 14 March 2017 diff hist +3‎ Luria-Bertani Media ‎No edit summary
20:0820:08, 14 March 2017 diff hist +142‎ N Luria-Bertani Media ‎ Created page with "Luria-Bertani Media Per liter: Tryptone (Shelf A) 10 grams Yeast Extract (Shelf A) 5 grams NaCl (Shelf A) 10 grams Adjust to desired pH."
20:0720:07, 14 March 2017 diff hist +25‎ Category:Protocols ‎No edit summary
20:0520:05, 14 March 2017 diff hist +630‎ N Creating Stock Cultures of Bacteria ‎ Created page with "Creating Stock Cultures of Bacteria # Heat an appropriate media (usually complex like LB, 100 mL) up to 37°C in an incubator. # Inoculate the media with bacteria. This is us..."
20:0420:04, 14 March 2017 diff hist +41‎ Category:Protocols ‎No edit summary
20:0220:02, 14 March 2017 diff hist −44‎ Plasmid Purification and Transformation Protocol ‎No edit summary
20:0120:01, 14 March 2017 diff hist −2‎ Plasmid Purification and Transformation Protocol ‎No edit summary
19:5919:59, 14 March 2017 diff hist +1,849‎ N Plasmid Purification and Transformation Protocol ‎ Created page with "Plasmid Purification/Transformation Protocol – JC 02/18/2016 Adapted from protocols found at “http://web.mnstate.edu/provost/” # Expand culture of bacteria overnight in..."
19:5719:57, 14 March 2017 diff hist +54‎ Category:Protocols ‎No edit summary
19:5619:56, 14 March 2017 diff hist +453‎ N Choosing a Plasmid ‎ Created page with "Choosing a Plasmid This step is necessary if your protein target does not come with a designated plasmid. Instead of steps, these are requirements for what should be located ..."
19:5519:55, 14 March 2017 diff hist +24‎ Category:Protocols ‎No edit summary
19:5419:54, 14 March 2017 diff hist +921‎ N Finding a Protein Target on the NESG website ‎ Created page with "Finding a Protein Target (on the NESG website) # Login: proteins Password: 2study # Start by focusing your search on a particular organism: human, myco, staph… # Begin by cl..."
19:5219:52, 14 March 2017 diff hist +50‎ Category:Protocols ‎No edit summary
19:5119:51, 14 March 2017 diff hist +478‎ N Chemical Disinfection of Glassware ‎ Created page with "Chemical Disinfection of Glassware Glassware and any other non-disposable item that comes into contact with biohazardous material (anything living) must be chemically disinfe..."
19:5019:50, 14 March 2017 diff hist +40‎ Category:Protocols ‎No edit summary
19:4919:49, 14 March 2017 diff hist −9‎ Autoclaving Laboratory Glassware and Media ‎No edit summary
19:4719:47, 14 March 2017 diff hist +1,788‎ N Autoclaving Laboratory Glassware and Media ‎ Created page with "Autoclaving Laboratory Glassware (and Media) Glassware that is to be used for cell growth must be sterilized beforehand. This is accomplished by first chemically disinfecting..."
19:4619:46, 14 March 2017 diff hist +1,239‎ N Filling a Magnet with Nitrogen ‎ Created page with "Filling a Magnet with Nitrogen # Find a rubber transfer line, two additional short pieces of rubber, and a glove. # Wheel the low pressure dewar up to the magnet to be filled..."
19:4419:44, 14 March 2017 diff hist +48‎ Category:Protocols ‎No edit summary
19:4419:44, 14 March 2017 diff hist +2‎ Category:Protocols ‎No edit summary
19:4219:42, 14 March 2017 diff hist +35‎ Category:Protocols ‎No edit summary
19:4119:41, 14 March 2017 diff hist −7‎ Changing the high pressure dewar ‎No edit summary
19:4019:40, 14 March 2017 diff hist +483‎ N Changing the high pressure dewar ‎ Created page with "Changing the High Pressure Dewar (on the 700) 1. Make sure that an experiment is not being collected on the 700. 2. Close the gas valve on the old/empty dewar. 3. Close the t..."
19:3819:38, 14 March 2017 diff hist +36‎ Category:Protocols ‎No edit summary
19:3819:38, 14 March 2017 diff hist −9‎ Gap sampling ‎No edit summary
19:3719:37, 14 March 2017 diff hist +9‎ Gap sampling ‎No edit summary
19:3519:35, 14 March 2017 diff hist −8‎ Category:Protocols ‎ Blanked the page
19:3519:35, 14 March 2017 diff hist −22‎ Test ‎ Blanked the page
19:3419:34, 14 March 2017 diff hist +22‎ N Test ‎ Created page with "category:Protocols"
19:3119:31, 14 March 2017 diff hist +8‎ Category:Protocols ‎No edit summary
19:2819:28, 14 March 2017 diff hist −37‎ Category:Protocols ‎ Blanked the page
19:2819:28, 14 March 2017 diff hist +37‎ Category:Protocols ‎No edit summary
18:4418:44, 14 March 2017 diff hist +49‎ N File:Changing the High Pressure Dewar.docx ‎ Instructions for changing the high pressure dewar current

20 March 2017

15 March 2017

14 March 2017