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Culture Passage Cells: Revision history

Culture Passage Cells: Revision history

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    21 July 2022

    • curprev 19:5219:52, 21 July 2022‎ Wiki Administrator talk contribs‎ 903 bytes +903‎ Created page with "==Culture and passage cells== * ready to passage when cell confluence to 80% or 90% or when the cell density reaches an average of 2 X 105 cells/cm2 * remove medium by aspiration * wash cells with PBS to remove the residual medium * add Trypsin-EDTA (enough to cover the flask surface) * incubate at 37oC for 5 minutes * observe by microscope to see cell death * add 2ml of medium trypsinization * pipet to detach all cells * transfer cell suspension to 15 ml tube..."
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