Collecting CEST Data: Difference between revisions

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# Click “execute” and hit “ok”.
# Click “execute” and hit “ok”.
# Carrier Positions
# Carrier Positions
*1H water pos. (O1) = 3290.987 Hz (this can be changed)
**1H water pos. (O1) = 3290.987 Hz (this can be changed)
*15N carrier pos = 118 ppm
**15N carrier pos = 118 ppm
*13C carrier pos = 118ppm (pseudo dimension)
**13C carrier pos = 118ppm (pseudo dimension)
# Click “Ok”
# Click “Ok”
# Calibrated pulse values:
# Calibrated pulse values:
*1H hard pulse = 10.25us @ -7.558748 dB (can be changed)
**1H hard pulse = 10.25us @ -7.558748 dB (can be changed)
*15N hard pulse = 35us @ -19.0309 dB
**15N hard pulse = 35us @ -19.0309 dB
*13C hard pulse = 12us @ -18.60338 dB
**13C hard pulse = 12us @ -18.60338 dB
# Click “Ok”
# Click “Ok”
# Values related to the weak B1 irradiation:
# Values related to the weak B1 irradiation:

Revision as of 21:29, 14 March 2017

Collecting CEST Data


  1. Open TopSpin >= 3.2.X
  2. Edit temperature
    1. “edte”
  3. Create a dataset and collect a NHSQC to check resolution and NS
  4. Type “edpy”, select n15cest_gb.py.
  5. Click “execute” and hit “ok”.
  6. Carrier Positions
    • 1H water pos. (O1) = 3290.987 Hz (this can be changed)
    • 15N carrier pos = 118 ppm
    • 13C carrier pos = 118ppm (pseudo dimension)
  1. Click “Ok”
  2. Calibrated pulse values:
    • 1H hard pulse = 10.25us @ -7.558748 dB (can be changed)
    • 15N hard pulse = 35us @ -19.0309 dB
    • 13C hard pulse = 12us @ -18.60338 dB
  1. Click “Ok”
  2. Values related to the weak B1 irradiation:
  • 15N weak B1 field = 25 Hz (can be changed)
  • Upfield irradiation offset limit = 100 ppm (can be changed)
  • Downfield irradiation offset limit = 140 ppm (can be changed)
  • Offset step size = 25 Hz (can be changed)
  1. Click “Ok”
  2. The experiment dataset will now be created. F1 is the pseudo dimension with the TD being the # of offsets that will be collected. The delays for these offsets can be found it FQLIST/FQ3LIST/n15cest_offset_std. This list name is overwritten each time a new CEST experiment is created so it is best to rename.
  3. Edit F3 and F2 TDs.
  4. DS must be >= 32.
  5. NS must be >= 2.
  6. Edit routing.
  7. Click “Edit” for CPDPRG, change 90x240y90x to 90x_240y_90x
  8. Make sure P50, P51, and P52 are all 500us
  9. Calculate receiver gain
    1. “rga”
  10. Edit D2 (t1 time)
  11. Must also edit D3 so that D3 > D2.
  12. Check ZGOPTNS
  13. “zg”
  14. The planes can be checked with “XFB”
    1. “xfb s23” plane 1
  15. Also, collecting the first plane is advisable.