Choosing a Plasmid

Choosing a Plasmid

This step is necessary if your protein target does not come with a designated plasmid. Instead of steps, these are requirements for what should be located on your plasmid.

1. Protein target sequence inserted
2. N- or C-terminal histidine tag of varying length
3. Antibiotic selection sequence- ampicillinm, kanamycin, or chloramphenicol.
4. Optional- his tag cleavage site. Either TEV or thrombin.
5. Optimized for expression in E. coli.