Category:Protein expression: Difference between revisions

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==Running SDS-PAGE==


#Make ready the samples to run
#Make ready the sample running buffer
#Make ready the gel
#Have the SDS-PAGE apparatus
#Place the the gel in the SDS-PAGE stand
#Place the stand with the gel in the SDS-PAGE apparatus bath
#Fill the space between the gels with running buffer
#Take off the combs of the gel (take it out gently)
#Load the sample to each well
#Do not use the wells at the left and right end of the gel
#Fill the SDS-PAGE apparatus bath with running buffer to the bottom of the gel
#Place the cover of the SDS-PAGE
##'''N.B'''  Make sure the correct plug is connected to the correct electrode (red goes to red and black goes to black)
#Plug the cables to the SDS-PAGE apparatus power source
#Turn of the SDS-PAGE power source
#Set to constant voltage
#Use 200 V
#Press the Run button to start the electrophoresis
##The voltage increases to 200 V from 0 V
##For 4% staking gel and 12.5 % separating gel the current reaches 50-60 mA and the power 10 -12 W
##The current and the power decreases in time but not reach zero (if it reaches zero see the troubleshoot)
##For the state type of gel it takes 35 - 40 min

Revision as of 14:26, 17 February 2014

Pages in category "Protein expression"

The following 3 pages are in this category, out of 3 total.