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| [[Category:Protocols]] | | [[category:Protein_Work]] |
| [[Category:Protein expression]]
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| ==Running SDS-PAGE==
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| #Make ready the samples to run
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| #Make ready the sample running buffer
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| #Make ready the gel
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| #Have the SDS-PAGE apparatus
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| #Place the the gel in the SDS-PAGE stand
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| #Place the stand with the gel in the SDS-PAGE apparatus bath
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| #fill the space between the gels with running buffer
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| #Take off the cumbs of the gel (take it out gently)
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| #Load the sample to each well
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| #Do not use the wells at the left and right end of the gel
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| #fill the SDS-PAGE apparatus bath with running buffer to the bottom of the gel
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| #Place the cover of the SDS-PAGE
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| ## '''N.B''' Make sure the correct plug is connected to the correct electrode (red goes to red and black goes to black)
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| #Plug the cables to the SDS-PAGE apparatus power source
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| #Turn of the SDS-PAGE power source
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| #Set to constant voltage
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| #Use 200 V
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| #Press the Run button to start the electrophoresis
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| ## The voltage increases to 200 V from 0 V
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| ##For 4% staking gel and 12.5 % separating gel the current reaches 50-60 mA and the power 10 -12 W
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| ##The current the the power decreases in time but not reach zero (if it reaches zero see the troubleshoot)
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| ##For the state type of gel it takes 35 - 40 min
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