Agarose Gel: Difference between revisions
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[[Category:Protocols]] | |||
[[Category:Protein expression]] | |||
==Preparing 1% Agarose Gel== | ==Preparing 1% Agarose Gel== | ||
Revision as of 19:58, 17 February 2014
Preparing 1% Agarose Gel
- Measure 0.6 g of agarose
- Pour the agarose powder into microwavable flask with 60 mL of 1XTAE buffer
- Microwave for 1- 3 min (until the agarose is completely dissolved)
- Cool down the agarose and add ethidium bromide to a final concentration of approximately 0.2 to 0.5 ug/mL
- Pour the agarose into a gel try with the well comb in place
- Let sit at room temperature for about half an hour
N.B Ethidium bromide is a known mutagen.